Abstract: 【Objectives】 To monitor the wildlife and habitats in Qilian Mountain National Park in an all-round, multi-dimensional, and multi-means way, master the distribution range and population dynamics of wildlife populations in Qilian Mountains, and analyze the spatial-temporal evolution of habitats. 【Methods】 Based on combing the research progress of wildlife monitoring at home and abroad, this study analyzed the deficiencies of existing wildlife monitoring in Qilian Mountain National Park; Based on the characteristics of wild animal resources and ecosystems in Qilian Mountain National Park, a comprehensive monitoring system for wild animals and their habitats was established by combining traditional and modern monitoring methods, integrating the monitoring methods and technologies of mammals, birds, fish, amphibians and reptiles, insects and habitats. A complement guarantee system was proposed for the construction of scientific research bases, monitoring data processing, information sharing, scientific research, talent teams, etc. 【Results】 The wildlife monitoring system proposed by this research can monitor the wildlife and its habitat in Qilian Mountains National Park in an all-round, multi-dimensional, and multi means way. At the same time, we should strengthen the construction of a comprehensive monitoring data information service platform, improve the scientific and technological support system, increase the management and protection of wildlife, enhance the ecological compensation system, and attach importance to the construction of talent teams to ensure the smooth implementation of wildlife monitoring in national parks, constantly advancing with the time. 【Conclusions】 The monitoring system for wild animals and their habitats in Qilian Mountain National Park proposed in this paper covered different animal groups, promoted the transformation of data into management strategies through an omnibearing security system, layed a scientific foundation for wildlife protection in Qilian Mountain National Park, and provided guidance for the formulation of wildlife protection and management strategies.