Abstract: As a carrier of renewable natural resources, grassland provides the material and environmental basis for sustainable regional development. Native landscape, grazing, and cultivated grasslands support the diversity of animal, plant, and microbial species. Functionally, grasslands serve crucial regulating, provisioning, supporting, and cultural inheritance roles. It is of great significance to construct a multi-functional target management model that focuses on the ecology, production, and livelihood function of grassland for protecting nature, severing residents, and sustainable development. Strengthening the construction of natural protected areas is conducive to the protection of biological diversity and indirectly helps implication of multi-functional grassland management objectives. Three-River Source National Park is the first and the largest national park in China. The National park is an important type of natural protected area system and its primary focus is ecological protection. Utilizing the multi-functional roles of grasslands to balance the contradiction between grassland ecological protection and economic development is the cornerstone of national park management.