Abstract: Forest health care is a way of health preservation for modern residents. Researches on forest health care have academic value and practical significance. The purpose of this study is to explore whether the five-sense therapy can improve the mental resilience of college students and alleviate negative emotions. In this study, 8 college students were selected as subjects to conduct an intervention study 6 times, once a week, and the psychological and physiological indexes were measured before and after each intervention. The mental resilience level of the subjects was measured with the Connor-Davidson Resilience Scale (CD-RISC). Negative emotions were measured by Depression Anxiety Stress Scale (DASS). The physiological indicators were measured by an electronic sphygmomanometer and fingertip pulse oximeter. Wilcoxon tests were used to analyze the changes in psychological resilience, negative emotion, and physiological indicators before and after the intervention of five-sense therapy. Analysis of variance for repeated measurement was performed to explore the time effects of the rising trend of psychological resilience. The results indicated that five-sense therapy could improve mental resilience. After the intervention of T4, T5, and T6, resilience was significantly higher than that before intervention (P<0.05). The time effects of the rising trend of resilience were statistically significant with F(6, 42)=8.361 and P<0.001. Five-sense therapy can reduce depression, anxiety, and stress. The effect of a multi-sensory experience environment on reducing negative emotion is better than that of a single sensory experience environment. Five-sense therapy can reduce systolic blood pressure and heart rate. Heart rate decreased significantly after each intervention from T1 to T6 (P<0.05), and the effect of five-sense therapy was more sensitive.