Abstract: Based on the general survey data of ancient and famous trees in Tonglu County of Zhejiang Province in 2016, the distribution characteristics, township distribution, growing places, tree species, and other resources of ancient and famous trees were analyzed. The spatial distribution law of ancient and famous trees in Tonglu County of Zhejiang Province was studied by using ArcGIS10.2 spatial analysis tool with the methods of the nearest neighbor index, nuclear density, concentration, and equilibrium analysis. The results showed that there were many famous trees, various species, and rich rare trees in Tonglu county of Zhejiang province. The main spatial concentration distribution was Tongjun street and Chengnan street. The corresponding geographical concentration index G is 32.10, which is larger than the ideal geographical concentration index. The research results can provide references for the protection of ancient and famous trees and the planning of urban development in Tonglu county of Zhejiang province.